Journal
MARINE BIOTECHNOLOGY
Volume 4, Issue 2, Pages 127-131Publisher
SPRINGER-VERLAG
DOI: 10.1007/s10126-001-0073-7
Keywords
confocal laser scanning microscopy; Schizothrix sp.; Solentia sp.; extracellular polymeric secretion; marine stromatolite; two-photon laser scanning microscopy
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The combination of a hydrophilic embedding resin, Nanoplast, with fluorescent probes, and subsequent imaging using two-photon and confocal laser scanning microscopy (2P-LSM and CLSM) has allowed in imaging of the in situ microspatial arrangements of microbial cells and their extracellular polymeric secretion (EPS) within marine stromatolites. Optical sectioning by 2P-LSM and CLSM allowed imaging of endolithic cyanobacteria cells, Solentia sp., seen within carbonate sand grains. 2P-LSM allowed very clear imaging with a high resolution of bacteria using DAPI, which normally require UV excitation and reduced photo-bleaching of fluorescent probes.
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