Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 13, Issue 2, Pages 167-183Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1163/156856202317414357
Keywords
poly(N-isopropylacrylamide); gelatin; thermoresponsiveness; cell adhesion; cell detachment
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Poly(N- isopropylacrylamide)-graft-copolymerized gelatin (PNIPAM-gelatin) was prepared by iniferter-based photopolymerization of multiply derivatized dithiocarbamylated gelatin. PNIPAM-gelatins exhibited low critical solution temperature (LCST) immediately below the physiological temperature, PNIPAAm-gelatin-coated dishes induced cell adhesion at 37degreesC but incomplete detachment at room temperature, whereas dishes coated with PNIPAM or a mixture of PNIPAAm and gelatin showed little cell adhesion. The mixture of PNIPAAm-gelatin and PNIPAAm induced cell adhesion at 37degreesC and detachment at 20degreesC: the degrees of cell adhesion and detachment depended on the mixed ratio of PNIPAAm-gelatin and PNIPAAm. Complete thermoresponsive adhesion and detachment were found for the mixture containing a small fraction of PNIPAAm-gelatin (approximately 5 wt% with respect to PNIPAAm gelatin content in the mixture is 2.7 wt%). Such a mixture may serve as thermoresponsive cell matrix for fabrication of a tissue-engineered device.
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