Journal
MOLECULAR BIOTECHNOLOGY
Volume 20, Issue 1, Pages 107-118Publisher
HUMANA PRESS INC
DOI: 10.1385/MB:20:1:107
Keywords
proteomics; nanoelectrospray mass spectrometry; tandem mass spectrometry; protein sequencing
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Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.
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