Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 948, Issue 1-2, Pages 193-201Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)01425-X
Keywords
electrochromatography; stationary phases, electrochromatography; polymer stationary phases; poly(glycidyl methacrylate-divinylbenzene) stationary phases; erythromycins; antibiotics; macrolide antibiotics
Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM020993, R37GM020993] Funding Source: NIH RePORTER
- NIGMS NIH HHS [GM 20993] Funding Source: Medline
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Monodisperse poly(glycidyl methacrylate-divinylbenzene) microspheres were prepared by a simple one-step dispersion polymerization process. Examination of the polymeric microspheres showed that they had a mean particle diameter of 3 mum and dual pore size distribution with mean pore diameters of 300 and 800 Angstrom. The microspheres were functionalized by introducing quaternary ammonium/octadecyl groups to obtain positively charged beads in a wide pH range. The functionalized beads were packed into fused-silica capillary having 50 pm inner diameter and used to separate erythromycin derivatives by capillary electrochromatography (CEC). These samples require gradient elution when separated by high-performance liquid chromatography (HPLC) or micro-HPLC, but with the new columns isocratic elution suffices for their separation by CEC. The column efficiency ranged from 40 000 to 50 000 theoretical plates. (C) 2002 Published by Elsevier Science B.V.
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