Journal
CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 39, Issue 1, Pages 71-76Publisher
TERMEDIA PUBLISHING HOUSE LTD
DOI: 10.5114/ceji.2014.42128
Keywords
juvenile idiopathic arthritis; Th17 cells; T-regulatory cells; proinflammatory cytokines
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Funding
- Medical University of Lodz: for the Department of Experimental Hematology [503/8-093-01/503-01]
- Medical University of Lodz: for the Department of Pediatric Cardiology and Rheumatology [502-03/8-000-01/502-64-055]
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Objectives: The imbalance between Th17 and T regulatory cells (Tregs) may be a key event in development of autoimmunity. The problem is poorly explored in juvenile idiopathic arthritis (JIA) so far. In this study, peripheral blood (PB) and synovial fluid (SF) Tregs and Th17 cells from were assessed in untreated JIA children. Material and methods: In 50 children with JIA the PB or SF percentages of Tregs and Th17 cells were assessed by flow cytometry, in comparison with PB Tregs and Th17 cells from 28 healthy controls. Additionally, in both groups the levels of proinfammatory cytokines, such as interleukin (IL)-1 beta, IL-6, IL-17, IL-21, IL-23 and tumor necrosis factor alpha (TNF-alpha) were assessed using ELISA method. Results: The proportion of JIA PB Th17 cells was significantly higher than in the controls (p=0.01). Serum levels of IL-1 beta, IL-6, IL-17, IL-23 were also significantly higher in JIA (p=0.011, p=0.007, p=0.008 and p=0.023, respectively). The highest serum IL-6 levels were observed in oligoarthritis JIA (p=0.031). Synovial fluid IL-21 concentration was distinctly higher in polyarticular JIA. Synovial fluid levels of TNF-a, IL-1 beta and IL-6 were significantly higher than in JIA PB (p=0.038, p=0.013 and p<0.001, respectively). There was a significant correlation between IL-6 and PB Tregs (p=0.02). Conclusions: The results of this comprehensive analysis indicate a role of Th17 cell activation in the pathogenesis of JIA.
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