Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 68, Issue 3, Pages 1192-1195Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.68.3.1192-1195.2002
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Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.
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