Journal
PLANT AND CELL PHYSIOLOGY
Volume 43, Issue 1, Pages 136-140Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcf014
Keywords
ABA; ABRE; bZIP protein; rice (Oryza sativa); stress
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The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGF-core of motif A, the principal ABRE of the rice gene, Osem. This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.
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