Journal
BIOMATERIALS
Volume 23, Issue 5, Pages 1383-1389Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0142-9612(01)00259-9
Keywords
titanium; surface modification; cell culture; morphological behavior; cell proliferation; collagen synthesis
Funding
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR046581] Funding Source: NIH RePORTER
- NIAMS NIH HHS [1R01AR46581] Funding Source: Medline
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In recent papers, we reported the results of a study on the graded porous titanium coatings on titanium by plasma spraying and amino-group ion implantation. The paper is to preliminarily evaluate the biocompatibility of surface-modified titanium through 2, 5 and 7 days cell culture in vitro. Cell morphology was observed by a scanning electron microscope. Cell proliferation and type I collagen synthesis were measured by 3(4,5-dimethyl-thiazole-2-yl)2,5-diphenyl tetrazolium bromide (MTT) and enzyme-linked immunosorbent assay (ELISA), respectively. Our experimental results showed that osteoblast-like cells attached and spread well on surface-modified titanium. Cells were observed to grow into the pores and form extracellular matrix. MTT and ELISA results showed no detrimental effect on the development of cell. These studies support the biocompatibility of surface-modified titanium. (C) 2002 Elsevier Science Ltd. All rights reserved.
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