Journal
CELLULOSE
Volume 16, Issue 3, Pages 501-517Publisher
SPRINGER
DOI: 10.1007/s10570-009-9274-4
Keywords
Plasma functionalization; Cellophane; alpha-Chymotrypsin immobilization; Spacer-chain molecules; Primary amine and carboxylic functionalities
Ask authors/readers for more resources
The surface functionalization process was accomplished in a consecutive 3 step process including: (1) Argon- and oxygen-plasma enhanced generation of free radical sites on cellophane surfaces; (2) In situ gas phase derivatization in the absence of plasma using hydrazine, ethylene diamine, or propylene diamine; (3) Second in situ, gas phase derivatization in the absence of plasma using oxallyl chloride or ex situ derivatization in the presence of glutaraldehyde. The presence of free radical sites on the plasma exposed cellophane surfaces was demonstrated using in situ sulfur dioxide and nitric oxide labeling techniques. It was shown that the free radical sites readily react under in situ conditions with the stable chain-precursor components and generate the desired spacer-chain molecules. ESCA, ATR-FTIR analysis and dying techniques were used to monitor the cellophane surface changes. A factorial design was used for selecting the optimal plasma parameters. Functionalized cellophane substrates were used for immobilization of alpha-chymotrypsin in the presence of spacer-chain molecules. The activity of the immobilized alpha-chymotrypsin was found to be lower in comparison to the activity of the free enzyme and the presence of virgin cellophane in the free enzyme solution also reduced significantly the activity of the enzyme. It is suggested that the swollen state of the cellophane plays a significant role in the decrease of the immobilized enzyme activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available