Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 372, Issue 1, Pages 155-163Publisher
SPRINGER-VERLAG BERLIN
DOI: 10.1007/s00216-001-1164-z
Keywords
inductively coupled plasma mass spectrometry; capillary electrophoresis; isotope dilution; metallothionein quantification
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In a new approach to the characterization and quantification of metallothionein isoforms an on-line isotope-dilution method in combination with the coupling of capillary electrophoresis (CE) to an inductively coupled plasma-sector field mass spectrometer (ICP-SFMS) is reported. Metallothionein (MT) isoforms are separated by CE and the elements Cu, Zn, Cd, and S are detected simultaneously by use of ICP-SFMS in the medium resolution mode. On-line isotope dilution is performed by continuous introduction of an isotopically enriched, species-unspecific spike solution after the separation step. MT from rabbit liver and a further purified MT-1 isoform were quantified by determination of sulfur, and the stoichiometric compositions of the metalloprotein complexes are characterized by determination of their sulfur-to-metal ratios.
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