Journal
CELLULAR SIGNALLING
Volume 25, Issue 11, Pages 2255-2262Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2013.07.010
Keywords
Oxidized-LDL; Cystathionine-gamma-Iyase; Hydrogen sulfide; JNK; Inflammation; Macrophage
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Funding
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Science and Education Methodist Youth Science and Technology Project of Suzhou Health Bureau [KJXW2011010]
- Start-up Funding for Imported Overseas Talents of Soochow University [Q421500210]
- National Natural Science Foundation of China [81171212, 81200894]
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Hydrogen sulfide (H2S), mainly produced by cystathionine gamma-lyase (CSE) in vascular system, emerges as a novel gasotransmitter exerting anti-inflammatory and anti-atherosclerotic effects. Alterations of CSE/H2S pathway may thus be involved in atherosclerosis pathogenesis. However, the underlying mechanisms are poorly understood. The present study showed that the levels of CSE mRNA and protein expression, as well as H2S production were decreased in ox-LDL-treated macrophage. CSE overexpression reduced the ox-LDL-stimulated tumor necrosis factor-alpha (TNF-alpha) generation in Raw264.7 and primary macrophage while CSE knockdown enhanced it Exogenous supplementation of H2S with NaHS and Na2S also decreased the production of TNF-alpha and intercellular adhesion molecule-1 (ICAM-1) in ox-LDL-stimulated macrophage, and alleviated the adhesion of macrophage to endothelial monolayer. Cysteine, a CSE preferential substrate for H2S biosynthesis, produced similar effects on the pro-inflammatory cytokine generation, which were reversed by CSE inhibitors PAG and BCA, respectively. Moreover, NaHS and Na2S attenuated the phosphorylation and degradation of I kappa B alpha and p65 nuclear translocation, as well as JNK activation caused by ox-LDL. The JNK inhibitor suppressed the NF-kappa B transcription activity in ox-LDL-treated cells. Furthermore, inhibitors of NF-kappa B (PDTC), ERK (U0126 and PD98059) and JNK (SP600125) partially blocked the suppression by ox-LDL on the CSE mRNA levels. Taken together, the findings demonstrate that ox-LDL may down-regulate the CSE/H2S pathway, which plays an anti-inflammatory role in ox-LDL-stimulated macrophage by suppressing JNK/NF-kappa B signaling. The study reveals new therapeutic strategies for atherosclerosis, based on modulating CSE/H2S pathway. (C) 2013 Elsevier Inc. All rights reserved.
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