Journal
BIOTECHNOLOGY LETTERS
Volume 24, Issue 5, Pages 355-358Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1014521021795
Keywords
ferrofluid-modified trypsin; magnetic fluids; trypsin inhibitors
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Ferrofluid-modified trypsin has been used for the detection and determination of selected xenobiotics that inhibit trypsin activity. The procedure is useful especially when colored samples or samples containing suspended solid impurities are to be assayed. Ferrofluid-modified trypsin was inhibited by Ag+ and Pb2+, selected dyes (safranin, thionin), bacitracin and 4-aminobenzamidine. Enzymes immobilized on magnetic particles can form a basis of new automated assay procedures for the determination of xenobiotics.
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