Journal
CELLULAR SIGNALLING
Volume 24, Issue 2, Pages 560-568Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2011.10.010
Keywords
Cytokines; Interleukins; Cardiac fibrosis; Myocardial remodeling; Migration; Proliferation; Signal transduction
Categories
Funding
- Veterans Affairs Office of Research and Development Biomedical Laboratory Research and Development Service [1IO1BX000246]
- NHLBI [HL-86787]
- [HL-70241]
- [HL-80682]
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The dual-specificity mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) inactivates MAP kinases by dephosphorylation. Here we show that the proinflammatory cytokine interleukin (IL)-17A induces adult mouse primary cardiac fibroblast (CF) proliferation and migration via IL-17 receptor A//IL-17 receptor C-dependent MKP-1 suppression, and activation of p38 MAPK and ERK1/2. IL-17A mediated p38 MAPK and ERK1/2 activation is inhibited by MKP-1 overexpression, but prolonged by MKP-1 knockdown. IL-17A induced miR-101 expression via PI3K/Akt, and miR-101 inhibitor reversed MKP-1 down regulation. Importantly, MKP-1 knockdown, pharmacological inhibition of p38 MAPK and ERK1/2, or overexpression of dominant negative MEK1, each markedly attenuated IL-17A-mediated CF proliferation and migration. Similarly, IL-17F and IL-17A/F heterodimer that also signal via IL-17RA/IL-17RC, stimulated CF proliferation and migration. These results indicate that IL-17A stimulates CF proliferation and migration via Akt/miR-101/MKP-1-dependent p38 MAPK and ERK1/2 activation. These studies support a potential role for IL-17 in cardiac fibrosis and adverse myocardial remodeling. (C) 2011 Elsevier Inc. All rights reserved.
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