Occurrence of trans-C-18 : 1 fatty acid isomers in goat milk: Effect of two dietary regimens

Trans-octadecenoic acid composition of goat milk fat was studied by using silver-ion thin layer chromatography combined with gas-liquid chromatography. This analytical procedure also was used to investigate the effect of diet on trans-C-18:1 fatty acid content in goat milk. Thirty-two goats were used in a 2 x 2 factorial arrangement and treatments. Two groups of goats received alfalfa hay at either a high or a low level of forage and two other groups received Rumiluz (dehydrated alfalfa from France Luzerne, Chalon en Champagne, France) at either a high or a low level of forage. Trans-C-18:1 isomer proportions (relative to total fatty acids) were, respectively, 2.02% for the Rumiluz low-level group and 1.75% for the Rumiluz high-level group versus 1.71% for the alfalfa low-level group and 1.21% for the alfalfa high-level group. Goats fed on Rumiluz thus produced relatively higher levels of trans-C-18:1 fatty acids than animals fed alfalfa hay. The results also showed that production of trans-C-18:1 fatty acids increased when the level of forage in the diet decreased. Moreover, goat milk trans-C-18:1 composition appeared similar to the cow milk profile. Vaccenic acid, trans-(18:1), was the major component and represented about 36.2% of total trans-C-18:1 isomers.