Journal
THEORETICAL AND APPLIED GENETICS
Volume 104, Issue 1, Pages 61-66Publisher
SPRINGER-VERLAG
DOI: 10.1007/s001220200007
Keywords
microsatellites; Vitis; mutation; flanking regions; ESTs
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Three EST-derived microsatellite loci from Vitis vinifera were amplified and sequenced across eight species of Vitaceae from four different genera. Phylogenetic analysis of the microsatellite's flanking regions produced informative results in congruence with previous studies. Generic relationships were respected and the data produced sufficient inter-specific variation to distinguish between Cayratia acris and Cayratia saponaria, two very closely related species. Overall, the sequence alignments showed that priming sites were conserved, whereas microsatellite repeats were present in most cases but structurally variable. The sequence data provided information on the evolutionary patterns of various microsatellite repeats and their correlation to evolutionary relationships among taxa.
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