Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 49, Issue 1, Pages 322-334Publisher
KARGER
DOI: 10.1159/000492883
Keywords
lncRNAs; Gastric cancer; EGFR-AS1; EGFR; MRNA stability; PI3K
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Funding
- Jiangsu Provincial Natural Science Foundation-Youth Foundation [BK20170699]
- Shandong Provincial Natural Science Foundation [ZR2015PH024]
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Background/Aims: LncRNA EGFR-AS1 is an antisense transcript of EGFR, which plays a key role in gastric cancer progression. This study was aimed to explore the effects of lncRNA EGFR-AS1 on GC and the underling mechanisms. Methods: The silencing of EGFR-AS1 expression was performed by using EGFR-AS1 shRNA lentivirus in MGC803 and SGC-7901 GC cell. The levels of lncRNA EGFR-AS1 and EGFR were detected by qPCR and western blot. Cell proliferation was assessed by CCK-8, EdU, and colony formation assays. The EGFR mRNA stability was explored by using RNA synthesis inhibitor alpha-amanitin. Results: In our study, EGFR-AS1 significantly up-regulated in GC tissues and correlated with tumor size. And the expression of EGFR-AS1 positively correlated with EGFR in tissues. Moreover, knock-down of EGFR-AS1 inhibited the proliferation of GC cells via suppressing EGFR-dependent PI3K/AKT pathway in vitro and in vivo. Mechanismly, depletion of EGFR-AS1 was found to decrease EGFR expression by reduction of EGFR mRNA stability. Conclusion: Our findings suggested that EGFR-AS1 might have an oncogenic effect on GC and serve as a potential target of GC. (C) 2018 The Author(s) Published by S. Karger AG, Basel.
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