Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 34, Issue 6, Pages 2266-2274Publisher
KARGER
DOI: 10.1159/000369669
Keywords
MicroRNA; Liver cancer cell; Migration; Invasion; Metastasis
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Funding
- National Natural Science foundation of China [81172592, 81270630]
- Science and Technology Special Project in Clinical Medicine of Jiangsu Province [BL2012056]
- 333 Project of Jiangsu Province [BRA2011085]
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Background: miR-378 regulates osteoblast differentiation and participates in tumor cell selfrenewal and chemo-resistance. However, the function of miR-378 in liver cancer cell migration has not been reported to date. Methods: miR-378 expression was examined using real-time quantitative PCR. HepG2 cell migration and liver cell invasion were examined using woundhealing and cell invasion assays. Additionally, HepG2 cell metastasis was analyzed in nude mice. Results: miR-378 over-expression enhances HepG2 cell proliferation, migration and liver cell invasion. Typical metastatic lesions were found in the livers of mice injected with miR-378transfected cells, and high levels of the CMV promoter were detected in the nodules, indicating that miR-378 promoted the metastasis of the tumor cells to the liver. We also demonstrated that miR-378 down-regulated Fus expression. Conclusions: Our results suggested that miR378 enhanced cell migration and metastasis by down-regulating Fus expression. Copyright (C) 2014 S. Karger AG, Basel
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