Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 31, Issue 4-5, Pages 649-658Publisher
KARGER
DOI: 10.1159/000350084
Keywords
Aquaporin 3; Aquaporin 7; Early embryo; Development
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Funding
- National Basic Foundation of China [2011CB944502, 2012CB944903]
- National Key Technology RD Program [2012BAI32B00]
- Public Welfare Technology Application Research Project of Zhejiang Province [2010C33167, 2010C13028]
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Background/Aims: Water channels, also named aquaporins (AQPs), play crucial roles in cellular water homeostasis. Methods: RT-PCR indicated the mRNA expression of AQPs 1-5, 7, 9, and 11-12, but not AQPs 0, 6, 8, and 10 in the 2 similar to 8-cell stage human embryos. AQP3 and AQP7 were further analyzed for their mRNA expression and protein expression in the oocyte, zygote, 2-cell embryo, 4-cell embryo, 8-cell embryo, morula, and blastocyst from both human and mouse using RT-PCR and immunofluorescence, respectively. Results: AQP3 and AQP7 were detected in all these stages. Knockdown of either AQP3 or AQP7 by targeted siRNA injection into 2-cell mouse embryos significantly inhibited preimplantation embryo development. However, knockdown of AQP3 in JAr spheroid did not affect its attachment to Ishikawa cells. Conclusion: These data demonstrate that multiple aquaporins are expressed in the early stage human embryos and that AQP3 and AQP7 may play a role in preimplantation mouse embryo development. Copyright (C) 2013 S. Karger AG, Basel.
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