Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 26, Issue 4-5, Pages 761-766Publisher
KARGER
DOI: 10.1159/000322343
Keywords
Phosphatidylserine; Scrambling; Calcium; Cell volume; Eryptosis; Apoptosis
Categories
Funding
- Carl-Zeiss-Stiftung
- IZKF-Promotionskolleg
- Deutsche Forschungsgemeinschaft [La 315/4-3, La 315/6-1]
Ask authors/readers for more resources
FTY720 is a potent anti-inflammatory drug known to trigger suicidal death or apoptosis of a variety of nucleated cells. Erythrocytes may similarly undergo suicidal erythrocyte death or eryptosis. Hallmarks of eryptosis include cell membrane scrambling and cell shrinkage, which are triggered by increase in cytosolic Ca2+ concentration and ceramide. The present study explored whether FTY720 stimulates eryptosis. Cell membrane scrambling was determined from annexin V-binding, cell shrinkage from forward scatter in FACS analysis, cytosolic Ca2+ concentration from Fluo3 fluorescence, ceramide formation from fluorescence-labeled antibody binding and hemolysis from the hemoglobin concentration in the supernatant. Within 48 hours exposure to FTY720 (10 mu M) significantly increased annexin V-binding, decreased forward scatter and increased cytosolic Ca2+ concentration but did not significantly modify ceramide formation. The effects of FTY720 were significantly blunted in the nominal absence of extracelluar Ca2+. In conclusion, at toxic concentrations, FTY720 stimulates suicidal cell death, an effect at least partially due to stimulation of Ca2+ entry. Copyright (C) 2010 S. Karger AG, Basel
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available