Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 93, Issue 2, Pages 316-325Publisher
WILEY
DOI: 10.1046/j.1365-2672.2002.01687.x
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Funding
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR013186] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM019698, R01GM019698] Funding Source: NIH RePORTER
- NCRR NIH HHS [RR 13186] Funding Source: Medline
- NIGMS NIH HHS [GM 19698] Funding Source: Medline
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Aims: To determine the mechanism of killing of Bacillus subtilis spores by hydrogen peroxide. Methods and Results: Killing of spores of B. subtilis with hydrogen peroxide caused no release of dipicolinic acid (DPA) and hydrogen peroxide-killed spores were not appreciably sensitized for DPA release upon a subsequent heat treatment. Hydrogen peroxide-killed spores appeared to initiate germination normally, released DPA and hydrolysed significant amounts of their cortex. However, the germinated killed spores did not swell, did not accumulate ATP or reduced flavin mononucleotide and the cores of these germinated spores were not accessible to nucleic acid stains. Conclusions: These data indicate that treatment with hydrogen peroxide results in spores in which the core cannot swell properly during spore germination. Significance and Impact of the Study: The results provide further information on the mechanism of killing of spores of Bacillus species by hydrogen peroxide.
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