Journal
ANALYST
Volume 127, Issue 3, Pages 396-400Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b108462n
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A novel hypoxanthine (Hx) microsensor was constructed. In this work, Nafion xanthine oxidase (XOD) and Au colloid were immobilized onto the surface of a Pt microelectrode. The enzyme biosensor displayed a quick and sensitive response to Hx. Under physiological conditions, a low detection limit, with high selectivity and sensitivity for Hx determination were obtained. The oxidation current [investigated using current-time (I-t) plots] was linear with Hx concentration ranging from 2.0x10(-7) to 2.0x10(-5) mol L-1 with a calculated detection limit of 1.0x10(-7) mol L-1 (S/N of 3). The biosensor should be promising for in vivo measurement of Hx without interferences and fouling. The change of Hx concentration in cardiac myocytes stimulated by L-arginine (L-Arg) and acetylcholine (Ach) was also studied.
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