Journal
MOLECULAR CELL
Volume 9, Issue 1, Pages 31-44Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(02)00436-7
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Funding
- NATIONAL CANCER INSTITUTE [P30CA033572] Funding Source: NIH RePORTER
- NCI NIH HHS [CA33572] Funding Source: Medline
- NIGMS NIH HHS [GM32627] Funding Source: Medline
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Pre-mRNA introns are spliced in a macromolecular machine, the spliceosome. For each round of splicing, the spliceosome assembles de novo in a series of ATP-dependent steps involving numerous changes in RNA-RNA and RNA-protein interactions. As currently understood, spliceosome assembly proceeds by addition of discrete U1, U2, and U4/U6.U5 snRNPs to a pre-mRNA substrate to form functional splicing complexes. We characterized a 45S yeast penta-snRNP which contains all five spliceosomal snRNAs and over 60 pre-mRNA splicing factors. The particle is functional in extracts and, when supplied with soluble factors, is capable of splicing pre-mRNA. We propose that the spliceosomal snRNPs associate prior to binding of a pre-mRNA substrate rather than with pre-mRNA via stepwise addition of discrete snRNPs.
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