Journal
MOLECULAR THERAPY
Volume 5, Issue 3, Pages 323-328Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/mthe.2002.0540
Keywords
gene transfer; electroporation; naked DNA; tissue damage
Categories
Funding
- NCI NIH HHS [CA74918] Funding Source: Medline
- NIAMS NIH HHS [AR45925] Funding Source: Medline
- NIDDK NIH HHS [DK54225, DK44935] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA074918] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [P01AR045925] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK054225, P01DK044935] Funding Source: NIH RePORTER
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Electroporation for gene delivery has attracted considerable attention recently, because of both the site-specific nature of the delivery and the high efficiency of the method. Electrotransfer of genes involves the application of an electric field to cells to enhance their permeability, facilitating exogenous polynucleotide transit across the cytoplasmic membrane. However, the relatively high electric field strength required for electroporation induces tissue damage, thus limiting its widespread application. We describe here a syringe electrode of our design, with which the same transfection efficiency can be achieved by using much lower electric field strength than that of conventional electrodes, such that the tissue damage is minimized.
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