Journal
PLANT MOLECULAR BIOLOGY
Volume 48, Issue 4, Pages 423-441Publisher
SPRINGER
DOI: 10.1023/A:1014047917226
Keywords
DNA-binding; ectopic expression; knotted1-like homeobox; Oryza sativa; promoter activity; shoot apical meristem
Categories
Ask authors/readers for more resources
Plant homeobox genes of the class 1 knox (knotted1-like) type are involved in the regulation of shoot apical meristem formation and function. Their expression generally occurs either throughout the meristem or specifically at the lateral organ boundaries. Down-regulation in the organ primordia is tightly controlled and misexpression in leaves leads to a perturbed development. Here, the transcriptional control of two rice knox genes, Oskn2 and Oskn3, was addressed, showing that the promoter sequences of both genes mediate the initial down-regulation during lateral organ formation, but are insufficient to keep expression in lateral organs stably off. Therefore, maintenance of the repressed state requires control elements outside the promoter regions. Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching, internode elongation and leaf patterning. However, small differences suggested that their target gene specificities are not completely overlapping. This was supported by the observation that Oskn3 protein but not Oskn2 could interact with two reported recognition sequences of a KNOX protein from barley. Finally, protein-protein interactions may contribute to the functioning of KNOX proteins, as the ability of Oskn3 and Oskn2 to form heterodimers could be demonstrated.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available