Journal
BIORESOURCE TECHNOLOGY
Volume 82, Issue 1, Pages 43-49Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0960-8524(01)00149-3
Keywords
Yarrowia lipolytica; industrial glycerol; lipid accumulation; microbial lipids
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Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol used as sole carbon substrate. Nitrogen-limited flask cultures were accompanied by restricted synthesis of reserve lipid, whilst amounts of citric acid were produced extracellularly. On the contrary, high amounts of reserve lipid (up to 3.5 g/l, 43% w/w of lipids in dry biomass) were produced in highly aerated continuous cultures, Lipid production was favoured at low specific dilution rates whilst fat-free material yield increased over the whole range of D (h(-1)). The maximum volumetric productivity obtained was 0.12 g lipid/1 h. Storage lipid composition did not present remarkable changes in the specific dilution rates tested. Oleate and linoleate were the dominant cellular fatty acids. () 2002 Published by Elsevier Science Ltd.
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