Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 79, Issue 3, Pages 257-259Publisher
WILEY
DOI: 10.1007/s11746-002-0470-1
Keywords
fractional crystallization; nutrition labeling; RP-HPLC; squalene; virgin olive oil
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A simple, rapid method for the determination of squalene in virgin olive oil was developed using RP-HPLC with detection at 208 nm. Fractional crystallization from methanol/acetone (7:3, vol/vol) was applied to obtain squalene in the liquid fraction of the oil prior to HPLC. Elution of squalene was then carried out isocratically with acetone/acetonitrile (40:60 vol/vol) within 11 min. The detection limit was 23 mg/kg, and the limit of quantification 79 mg/kg. The precision of the crystallization procedure (CV% = 3.76, n = 7) and the mean recovery (92.5 and 81.5% for the 7,000 and 700 mg/kg levels of addition, respectively) were satisfactory. The method is easily applicable to fulfill future needs for nutrition labeling.
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