Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures

In a suspension culture of Vitis vinifera cells, anthocyanin biosynthesis was enhanced, whereas the cell growth was inhibited, following either the addition of jasmonic acid or light irradiation. The maximum anthocyanin accumulation of 13.8 CV (color value)/g-FCW (fresh cell weight) occurred on day 7 when jasmonic acid was added to the cultures at a final concentration of 20 muM on day 0. This represented an 8.5-fold increase compared with the control culture in the dark. Following the continuous light irradiation of 8000-8300 lux, the maximum anthocyanin accumulation reached was 6.8 CV/g-FCW on day 10, which was 4.8-fold that of the control. A process that combined jasmonic acid treatment and light irradiation resulted in a significant synergistic enhancement of anthocyanin accumulation up to 22.62 CV/g-FCW on day 7. This value was 13.9-fold that of the control. As a result, the maximum anthocyanin production of 2200 CV/l was achieved on day 10, representing a 5.8-fold increase compared with the control. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.