Journal
CELLULAR IMMUNOLOGY
Volume 279, Issue 2, Pages 124-133Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2012.10.007
Keywords
Intestinal intraepithelial lymphocytes (IELs); Intestinal epithelial cells (IECs); FK506; Turnover; Alkaline phosphatase; CD98; Microvilli
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [15603007]
- Grants-in-Aid for Scientific Research [15603007] Funding Source: KAKEN
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To elucidate the interaction of intestinal intraepithelial lymphocytes (IELs) with intestinal epithelial cells (IECs), we investigated alterations of IECs by activating or inactivating IELs. The stimulation of IELs with anti-mouse CD3 monoclonal antibody induced massive apoptosis of IECs. Changes in IECs and IELs from mice that received daily administration of FK506 for 14 days were investigated. IELs, particularly TCR-gamma delta(+) IELs, were reduced in cell number, and a decrease of cytotoxic activity was observed. Under this condition, loss of apoptotic cells at the tips of villi and delayed turnover of IECs were detected. The expressions of alkaline phosphatase and CD98 amino acid transporters on IECs were decreased. Furthermore, abnormal skeletal organization of villi and weakened binding of IECs to the basement membrane were shown. These results suggest that inactivated IECs, which should be led to apoptosis, remained. It was strongly suggested that IELs participated in IEC turnover. (C) 2012 Elsevier Inc. All rights reserved.
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