Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 25, Issue 20, Pages 3099-3116Publisher
TAYLOR & FRANCIS INC
DOI: 10.1081/JLC-120016211
Keywords
monoliths; short columns; chromatographic columns; stationary phases; HETP; Z factors
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Convective Interaction Media(R) (CIM) disk monolithic columns are specific among the chromatographic columns because of their monolithic structure and extremely short column length. In this work, HETP values and Z factors for different groups of molecules-proteins, DNA, oligonucleotides, peptides, and organic acids on strong anion exchange CIM disk monolithic columns were determined. Results are discussed in terms of the molecule structures and applied to develop different approaches for successful separation of abovementioned group of molecules on these types of columns.
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