Journal
CELLULAR AND MOLECULAR NEUROBIOLOGY
Volume 32, Issue 3, Pages 353-360Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10571-011-9763-5
Keywords
Isorhynchophylline; Alzheimer's disease; beta-Amyloid; PC12 cells; Neuroprotection
Categories
Funding
- The Chinese University of Hong Kong [2030409]
Ask authors/readers for more resources
Beta-amyloid peptide (A beta), a major protein component of senile plaques, has been considered as a critical cause in the pathogenesis of Alzheimer's disease (AD). Modulation of the A beta-induced neurotoxicity has emerged as a possible therapeutic approach to ameliorate the onset and progression of AD. The present study aimed to evaluate the protective effect of isorhynchophylline, an oxindole alkaloid isolated from a Chinese herb Uncaria rhynchophylla, on A beta-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. The results showed that pretreatment with isorhynchophylline significantly elevated cell viability, decreased the levels of intracellular reactive oxygen species and malondialdehyde, increased the level of glutathione, and stabilized mitochondrial membrane potential in A beta(25-35)-treated PC12 cells. In addition, isorhynchophylline significantly suppressed the formation of DNA fragmentation and the activity of caspase-3 and moderated the ratio of Bcl-2/Bax. These results indicate that isorhynchophylline exerts a neuroprotective effect against A beta(25-35)-induced neurotoxicity in PC12 cells, at least in part, via inhibiting oxidative stress and suppressing the mitochondrial pathway of cellular apoptosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available