Journal
ANALYTICAL LETTERS
Volume 35, Issue 7, Pages 1149-1162Publisher
MARCEL DEKKER INC
DOI: 10.1081/AL-120005969
Keywords
multiplex PCR; uniform design; optimization; human carcinoma virus
Categories
Ask authors/readers for more resources
Multiplex PCR is proposed for simultaneous detection of the conserved genes of six human carcinoma-related viruses, which include HBV, HCV, EBV, HCMV, HSV-1 and HIV-1. The final concentration of various primers, which is one of the key factors affecting simultaneous amplification, was adjusted by using uniform design. Four loci, five loci and six loci of the target genes were successfully co-amplified. The optimized primer combination was applied to amplify viral genomes from blood sample or tissue culture. The method is to be developed further for clinical diagnosis and blood quality control.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available