Journal
MOLECULAR IMMUNOLOGY
Volume 38, Issue 11, Pages 817-824Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0161-5890(02)00004-4
Keywords
monocyte differentiation; monocytic activation receptor complexes; TREM-1; MDL-1; DAP12; gene regulation
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Funding
- NATIONAL CANCER INSTITUTE [U01CA080200] Funding Source: NIH RePORTER
- NCI NIH HHS [U01 CA080200] Funding Source: Medline
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The triggering receptor expressed on myeloid cells (TREM-1) and the myeloid DAP12-associating lectin (MIDL-1) are two recently identified receptors which associate non-covalently with DAP12 to form receptor complexes involved in monocytic activation and inflammatory response. In this study, we investigated whether the expression of TREM-1, MDL-1, and DAP12 correlated with myelomonocytic differentiation. Northern and RT-PCR revealed a strong expression of TREM-1, MDL-1, and DAP12 in peripheral blood-derived CD14(+) mature monocytes in contrast to undifferentiated bone marrow CD34(+) stem cells, and in the differentiated versus undifferentiated U937 cells. TREM-1 and MDL-1 RNA expression was also more elevated in adult than fetal tissues and in normal than malignant cells. These findings suggest that the TREM-1/DAP12 and MDL-1/DAP12 signaling pathways are features of mature differentiated myelomonocytic cells. In addition, expression of an alternative mRNA TREM-1 splice variant (TREM-1sv) was detected that might translate into a soluble receptor with potential as a regulator of myeloid activation. (C) 2002 Elsevier Science Ltd. All rights reserved.
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