Journal
EXPERIMENTAL EYE RESEARCH
Volume 74, Issue 1, Pages 131-139Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1006/exer.2001.1109
Keywords
corneal transplantation; CD80; CD86; costimulatory signal; allogeneic rejection
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The purpose of this study was to investigate the role of CD80 and CD86 costimulatory molecules in corneal allograft rejection. Anti-CD80 and anti-CD86 monoclonal antibodies (mAbs) were administered after orthotopic corneal allograft transplantation. Graft rejection was observed by biomicroscopy. Population and localization of CD80(+) and CD86(+) cells in the cornea, cervical lymph nodes, and spleen were examined by flow cytometry and immunohistochemistry, The combined use of anti-CD80 and anti-CD86 mAbs was effective in prolonging corneal allograft survival. In the untreated mice bearing rejected graft, many CD86(+) and CD80(+) cells were found around the host-graft junctional area in the cornea. and CD86 high cells were found in the cervical lymph node and spleen. In contrast, few CD86(+) or CD80(+) cells were observed in the cornea, cervical lymph node, and spleen from the mice treated with antiCD80/CD86 mAbs. These results demonstrated a significant role of CD80 and CD86 costimulatory molecules in corneal allograft rejection. (C) 2002 Elsevier Science Ltd.
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