Journal
CELLULAR AND MOLECULAR LIFE SCIENCES
Volume 67, Issue 22, Pages 3915-3925Publisher
SPRINGER BASEL AG
DOI: 10.1007/s00018-010-0420-9
Keywords
Cancer stem cells; Ovarian carcinoma; CD44; CD24
Categories
Funding
- National Natural Science Foundation of China [30672230]
- National Program on Key Basic Research Project of China [2009CB521800]
- Foundation of Health Department of Zhejiang Province of China [2007A129]
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Cancer stem cells (CSCs) play an important role in the development, invasion, and drug resistance of carcinoma, but the exact phenotype and characteristics of ovarian CSCs are still disputable. In this study, we identified cancer stem cell-like cells (CSC-LCs) and investigated their characteristics from the ovarian adenocarcinoma cell line 3AO. Our results showed that CSC-LCs were enriched in sphere-forming test and highly expressed CD44(+)CD24(-). The spheres and CD24(-) cells possessed strong tumorigenic ability by transplantation into nonobese diabetic/severe combined immunodeficient mice. CD44(+)CD24(-) cells expressed stem cell markers and differentiated to CD44(+)CD24(+) cells by immunofluorescence assay and fluorescence-activated cell-sorting analysis. In vitro experiments verified that CD44(+)CD24(-) cells were markedly resistant to carboplatin and paclitaxol. In conclusion, our study identifies the CD44(+)CD24(-) phenotype, self-renewal, high tumorigenicity, differentiation potential, and drug resistance of ovarian CSC-LCs. Our findings may provide the evidence needed to explore a new strategy in the treatment of ovarian cancer.
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