Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 13, Issue 1, Pages 99-106Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1044-0305(01)00333-6
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Funding
- NCI NIH HHS [CA81654] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R21CA081654, R33CA081654] Funding Source: NIH RePORTER
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One of the key qualities of mass spectrometric measurements for biomolecules is the mass measurement accuracy (MMA) obtained. FTICR presently provides the highest MMA over a broad m/z range. However, due to space charge effects, the achievable MMA crucially depends on the number of ions trapped in the ICR cell for a measurement. Thus, beyond some point, as the effective sensitivity and dynamic range of a measurement increase, MMA tends to decrease. While analyzing deviations from the commonly used calibration law in FTICR we have found systematic errors which are not accounted for by a global space charge correction approach. The analysis of these errors and their dependence on charge population and post-excite radius have led us to conclude that each ion cloud experiences a different interaction with other ion clouds. We propose a novel calibration function which is shown to provide an improvement in MMA for all the spectra studied. (J Am Soc Mass Spectrom 2002, 13, 99-106) (C) 2002 American Society for Mass Spectrometry.
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