Journal
HUMAN MUTATION
Volume 19, Issue 4, Pages 410-415Publisher
WILEY-LISS
DOI: 10.1002/humu.10073
Keywords
padlock probes; DNA ligation; SNP; in situ; mutation detection; RNA analysis
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Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling-circle replication, as well as to sort reaction products on tag-arrays. We argue that the probes have the potential to render high-throughput genetic analyses precise and affordable. Hum Mutat 19:410-415, 2002. (C) 2002 Wiley-Liss, Inc.
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