Journal
CELLULAR AND MOLECULAR LIFE SCIENCES
Volume 67, Issue 5, Pages 829-840Publisher
SPRINGER BASEL AG
DOI: 10.1007/s00018-009-0218-9
Keywords
Aquaporin; Glial cells; Water permeability; Regulation; Protein kinase C; Isoforms
Categories
Funding
- Nordic Centre of Excellence in Water-Imbalance Related Disorders
- Lundbeck Foundation
- Danish Medical Research Council
- E. Danielsen's Foundation
- Augustinus Foundation
- Michaelsen's Foundation
- Novo Nordisk Foundation
- Carlsberg Foundation
- L'Oreal/UNESCO/Royal Danish Academy of Sciences Scholarship to Young Women in Science
- HeartLung Foundation
- Functional genomics program of the Norwegian Research Council, FUGE
- Marie Curie Intra-European Fellowship
- Danish National Research Foundation
Ask authors/readers for more resources
Aquaporin 4 (AQP4) is expressed in the perivascular glial endfeet and is an important pathway for water during formation and resolution of brain edema. In this study, we examined the functional properties and relative unit water permeability of three functional isoforms of AQP4 expressed in the brain (M1, M23, Mz). The M23 isoform gave rise to square arrays when expressed in Xenopus laevis oocytes. The relative unit water permeability differed significantly between the isoforms in the order of M1 > Mz > M23. None of the three isoforms were permeable to small osmolytes nor were they affected by changes in external K+ concentration. Upon protein kinase C (PKC) activation, oocytes expressing the three isoforms demonstrated rapid reduction of water permeability, which correlated with AQP4 internalization. The M23 isoform was more sensitive to PKC regulation than the longer isoforms and was internalized significantly faster. Our results suggest a specific role for square array formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available