Journal
CELLULAR AND MOLECULAR LIFE SCIENCES
Volume 66, Issue 3, Pages 516-525Publisher
SPRINGER BASEL AG
DOI: 10.1007/s00018-008-8601-5
Keywords
Melatonin receptors; RORalpha; cAMP; melatonin; MT1
Categories
Funding
- Ministerio de Sanidad FIS [06/ 0091]
- Red-FIS [RD06/0013/0001]
- Grupo Excelencia del Plan Andaluz de Investigacion [P06-CTS-01604]
Ask authors/readers for more resources
Human lymphocyte melatonin, through membrane and nuclear receptors binding, acts as an activator in IL-2 production. Antagonism of membrane melatonin receptors using luzindole exacerbates the drop of the IL-2 production induced by PGE(2) in peripheral blood mononuclear and Jurkat cells. This paper studies the melatonin membrane and nuclear receptors interplay in PGE(2)-diminished IL-2 production. The decrease in IL-2 production after PGE(2) and/or luzindole administration correlated with downregulation in the nuclear receptor ROR alpha. We also highlighted a role of cAMP in the pathway, because forskolin mimicked the effects of luzindole and/or PGE(2) in the ROR alpha expression. Finally, a significant ROR alpha downregulation was observed in T cells permanently transfected with inducible MT1 antisense. In conclusion, we show a novel connection between melatonin membrane receptor signalling and ROR alpha expression, opening a new way to understand melatonin regulation in lymphocyte physiology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available