Journal
CELLULAR AND MOLECULAR BIOENGINEERING
Volume 7, Issue 3, Pages 293-306Publisher
SPRINGER
DOI: 10.1007/s12195-014-0342-y
Keywords
Lamin; Nucleus; Mechanics; Cancer; Microfluidics; Microstructures; Metastasis; Invasion; Nuclear envelope
Funding
- National Science Foundation [ECCS-0335765, CBET-1254846]
- National Institutes of Health [R01 NS059348, R01 HL082792]
- Department of Defense Breast Cancer Idea Award [BC102152]
- Cornell Center on the Microenvironment & Metastasis through National Cancer Institute
- CDMRP [BC102152, 545346] Funding Source: Federal RePORTER
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1254846] Funding Source: National Science Foundation
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Cell motility plays a critical role in many physiological and pathological settings, ranging from wound healing to cancer metastasis. While cell migration on 2-dimensional (2-D) substrates has been studied for decades, the physical challenges cells face when moving in 3-D environments are only now emerging. In particular, the cell nucleus, which occupies a large fraction of the cell volume and is normally substantially stiffer than the surrounding cytoplasm, may impose a major obstacle when cells encounter narrow constrictions in the interstitial space, the extracellular matrix, or small capillaries. Using novel microfluidic devices that allow observation of cells moving through precisely defined geometries at high spatial and temporal resolution, we determined nuclear deformability as a critical factor in the cells' ability to pass through constrictions smaller than the size of the nucleus. Furthermore, we found that cells with reduced levels of the nuclear envelope proteins lamins A/C, which are the main determinants of nuclear stiffness, passed significantly faster through narrow constrictions during active migration and passive perfusion. Given recent reports that many human cancers have altered lamin expression, our findings suggest a novel biophysical mechanism by which changes in nuclear structure and composition may promote cancer cell invasion and metastasis.
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