Journal
CELLULAR & MOLECULAR IMMUNOLOGY
Volume 10, Issue 6, Pages 490-496Publisher
CHIN SOCIETY IMMUNOLOGY
DOI: 10.1038/cmi.2013.33
Keywords
genotyping techniques; group-specific amplification; HLA-A*02 antigen; mono-allelic sequencing; sequence-based typing
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Funding
- National Natural Science Foundation of China [NSF-30830093]
- National Key Program (973) for Basic Research of China [2009CB522409]
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HLA-A*02 is the most prevalent and polymorphic major histocompatibility complex (MHC) allele family in humans. Functional differences have been revealed among subtypes, demanding further subtyping of HLA-A*02 in basic and clinical settings. However, the fast growing polymorphisms render traditional primer-or probe-based typing methods impractical and result in increasing ambiguities in direct sequence-based typing. In this study, we combined group-specific amplification and mono-allelic sequencing to design and validate a simple scheme for the complete screening and accurate subtyping of all 540 reported HLA-A*02 alleles. This scheme could be performed in routine labs to facilitate studies with an interest in HLA-A*02.
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