Journal
CELLULAR & MOLECULAR IMMUNOLOGY
Volume 7, Issue 4, Pages 296-305Publisher
CHIN SOCIETY IMMUNOLOGY
DOI: 10.1038/cmi.2010.15
Keywords
adenovirus; B lymphoma; gene transfer; LIGHT/TNFSF14
Categories
Funding
- National Natural Science Foundation of China [30328011, 30872377]
- National Basic Research Program of China [2004CB518802]
- Science and Technology Department of Zhejiang Province [2008C23044]
Ask authors/readers for more resources
Here, we investigated the antitumor effect of adenovirus-mediated gene transfer of LIGHT, the tumor-necrosis factor (TNF) superfamily member also known as TNFSF14, in the murine A20 B-cell lymphoma. LIGHT gene modification resulted in upregulated expression of Fas and the accessory molecule-intercellular adhesion molecule-1 (ICAM-1) on A20 cells and led to enhanced A20 cell apoptosis. LIGHT-modified A20 cells effectively stimulated the proliferation of T lymphocytes and interferon (IFN)-gamma production in vitro. Immunization of BALB/c mice with a LIGHT-modified A20 cell vaccine efficiently elicited protective immunity against challenge with the parental tumor cell line. Adenovirus-mediated gene transfer of LIGHT by intratumoral injection exerted a very potent antitumor effect against pre-existing A20 cell lymphoma in BALB/c mice. This adenovirus-mediated LIGHT therapy induced substantial splenic natural killer (NK) and cytotoxic T lymphocyte (CTL) activity, enhanced tumor infiltration by inflammatory cells and increased chemokine expression of CC chemokine ligand 21 (CCL21), IFN-inducible protein-10 (IP-10) and monokine induced by IFN-gamma (Mig) from tumor tissues. Thus, adenovirus-mediated LIGHT therapy might have potential utility for the prevention and treatment of B-cell lymphoma. Cellular & Molecular Immunology (2010) 7, 296-305; doi:10.1038/cmi.2010.15; published online 26 April 2010
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available