Journal
CELLS TISSUES ORGANS
Volume 195, Issue 1-2, Pages 5-14Publisher
KARGER
DOI: 10.1159/000331412
Keywords
Smooth muscle cells; Neural crest; Embryonic stem cells; Induced pluripotent stem cells
Funding
- National Institutes of Health [EB012240, HL083900]
- California Institute for Regenerative Medicine [TG2-01164]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL083900] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB012240] Funding Source: NIH RePORTER
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The heterogeneity of vascular smooth muscle cells (SMCs) is related to their different developmental origins such as the neural crest and mesoderm. Derivation of SMCs from different origins will provide valuable in vitro models for the investigation of vascular development and diseases. From the perspective of regenerative medicine and tissue engineering, an expandable cell source of SMCs is required for the construction of tissue-engineered blood vessels. In this study, we developed a robust protocol to derive neural crest stem cells (NCSCs) from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). NCSCs derived from ESCs and iPSCs were expandable with similar cell doubling times. NCSCs were capable of differentiating into neural and mesenchymal lineages. TGF-beta 1 induced the expression of SMC markers calponin-1, SM22 alpha, and smooth muscle myosin heavy chain and resulted in the assembly of smooth muscle alpha-actin, calponin-1, and SM22 alpha into stress fibers. This work provides a basis for using iPSCs to study SMC biology and deriving vascular cells for tissue engineering. Copyright (C) 2011 S. Karger AG, Basel
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