Journal
CELLS TISSUES ORGANS
Volume 191, Issue 5, Pages 357-364Publisher
KARGER
DOI: 10.1159/000276589
Keywords
Stem cells; Apical papilla; Immunogenicity; Immunosuppression; Tissue engineering; Minipig
Funding
- National Basic Research Program of China [2007CB947304, 2010CB944801]
- Jurisdiction of Beijing Municipality [PHR20090510]
- Beijing Major Scientific Program [D0906007000091]
- National Natural Science Foundation of China [30428009, 30700941]
- Beijing New Star Program [2007B067]
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The use of allogeneic stem cells strongly extends the range of stem cell applications in dentistry; however, immunological rejection remains a major concern. There is little information about the immunological features of dental-related stem cells in the literature. Therefore, we investigated the immunological characteristics of stem cells from the root apical papilla (SCAP) of swine in vitro by measuring T cell immunomodulation and apoptosis. We found that SCAP expressed a low level of swine leukocyte antigen (SLA) class I molecules and were negative for SLA class II DR molecules. Moreover, SCAP could inhibit autologous T cell proliferation stimulated by phytohemagglutinin (PHA) and a one-way mixed lymphocyte reaction in a dose-dependent manner. In addition, SCAP could suppress proliferation of allogeneic T cells in a dose-dependent manner, with or without mitomycin C pretreatment. Moreover, soluble factor(s) may be in-volved in the SCAP-mediated immune suppression. After a 5-day coculture of SCAP, allogeneic T cells, and PHA, only 1.22% of T cells were apoptotic. These data indicated that SCAP were weakly immunogenic and suppressed T cell proliferation in vitro through an apoptosis-independent mechanism. Copyright (c) 2010 S. Karger AG, Basel
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