Carbon isotopic fractionation by Archaeans and other thermophilic prokaryotes

This study of carbon isotopic fractionation in a wide array of 21 phylogenetically diverse microbial species provides an opportunity to correlate carbon isotopic fractionations with a biochemical pathway. These carbon isotopic fractionation experiments included two members of the Aquificales and two members of the Thermoproteales using the reductive TCA cycle, three members of the Sulfolobales using the 3-hydroxypropionate cycle, as well as three Archaeoglobales and seven methanogens using the acetyl-CoA pathway. In these experiments, microorganisms using the reductive tricarboxylic acid cycle (with 6 values between 2.0 and 5.5parts per thousand) and the 3-hydroxypropionate cycle (with epsilon values between 0.2 and 3.6parts per thousand) demonstrated significantly less carbon isotopic fractionation than methanogens using the acetyl-CoA pathway. The results reported here for the acetyl-CoA pathway-utilizing microbes, however, vary over a remarkably wide range with epsilon values of 2.7 to 8.0parts per thousand for the Archaeoglobales and epsilon values of 4.8 to 26.7parts per thousand for the methanogens. The magnitude of carbon isotopic fractionation observed in species of Methanococcus were related to the particular growth status that had been attained by the various cultures, with increasing isotopic fractionation as growth proceeded. (C) 2002 Elsevier Science Ltd. All rights reserved.