Journal
MICROBIOLOGY AND IMMUNOLOGY
Volume 47, Issue 8, Pages 613-617Publisher
CENTER ACADEMIC PUBL JAPAN
DOI: 10.1111/j.1348-0421.2003.tb03424.x
Keywords
rabies virus; reverse genetics; T7 RNA polymerase-expressing BHK cells; internal ribosomal entry site
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To improve efficiency of recovery of rabies virus from cloned cDNA, we established a BHK cell clone that stably expresses T7 RNA polymerase, which we named BHK/T7-9. We also constructed new helper plasmids for expression of nucleoprotein and RNA polymerase of the RC-HL strain using the pTM1 plasmid vector, which makes the T7 RNA polymerase-transcripts from the plasmid cap-independent for translation. After co-transfection of these helper plasmids and the previously constructed full-length genome plasmid of the RC-HL strain to BHK/T7-9 cells, recombinant rabies virus was efficiently recovered from the cloned cDNA.
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