Journal
JOURNAL OF INSECT PHYSIOLOGY
Volume 49, Issue 1, Pages 53-61Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0022-1910(02)00245-7
Keywords
immunohistochemistry; competitive ELISA; AKH level; CNS; haemolymph; wing polymorphism; Pyrrhocoris
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We have used an enzyme-linked immunoassay (ELISA) for determination of the AKH content in CNS (brain + corpora cardiaca + corpora allata) and haemolymph of adult macropterous and brachypterous females of the bug Pyrrhocoris apterus. The tests revealed that the AKH content fluctuates between 1 and almost 4 pmol/CNS during the first 14 days of adult life and significantly increased in order diapausing brachypters
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