Journal
GENOME BIOLOGY
Volume 4, Issue 12, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/gb-2003-4-12-r82
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Funding
- NATIONAL CANCER INSTITUTE [Z01BC007349] Funding Source: NIH RePORTER
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Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.
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