Journal
CELL TRANSPLANTATION
Volume 19, Issue 6-7, Pages 701-706Publisher
SAGE PUBLICATIONS INC
DOI: 10.3727/096368910X508799
Keywords
Cryopreservation; Human hepatocytes; Sericin
Funding
- Japan Health Sciences Foundation, Tokyo, Japan [KH71066, KHD1027]
- Japan Science Society
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The methodology of cryopreservation of human hepatocytes remains unsatisfactory. Even when the viability of thawed cells is tolerable, the cells often lose the attaching capability to a culture dish, resulting in the cells' inability to survive. Previously, we described the effectiveness of maltose on the attachment of hepatocytes. This article demonstrates that a silk-derived high molecular protein, sericin, improves the cell-attaching capability in the serum-free freezing medium. When human hepatocytes [initial viability: 60.9 +/- 3.1% (mean +/- SD, n = 3)] were frozen with serum-free Dulbecco's modified Eagle medium (DMEM) containing 10% dimethyl sulfoxide (DMSO), the viability was 29.4 +/- 3.2% and the cell-attaching capability 20.4 +/- 4.1%. On the other hand, DMEM containing 10% DMSO and 1% sericin increased the values to 45.0 +/- 0.8% and 26.2 +/- 3.2%. Moreover, the addition of 0.1 mol/L maltose to the sericin-containing medium improved to 42.2 +/- 3.2% and 51.1 +/- 1.0%, as we demonstrated in a previous report. The present results indicated that sericin combined with maltose is a novel additive in the serum-free freezing medium for human hepatocytes.
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