Journal
CELL TRANSPLANTATION
Volume 19, Issue 12, Pages 1523-1535Publisher
SAGE PUBLICATIONS INC
DOI: 10.3727/096368910X515872
Keywords
Islet culture; Surface modification; Encapsulation; Scaffolds; Bioreactors
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Postisolation islet survival is a critical step for achieving successful and efficient islet transplantation. This involves the optimization of islet culture in order to prolong survival and functionality in vitro. Many studies have focused on different strategies to culture pancreatic islets in vitro through manipulation of culture media, surface modified substrates, and the use of various techniques such as encapsulation, embedding, scaffold, and bioreactor culture strategies. This review aims to present and discuss the different methodologies employed to optimize pancreatic islet culture in vitro as well as address their respective advantages and drawbacks.
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