Journal
CELL TRANSPLANTATION
Volume 18, Issue 4, Pages 423-431Publisher
SAGE PUBLICATIONS INC
DOI: 10.3727/096368909788809811
Keywords
Mesenchymal stem cells; Stem cell-based therapy; Retinal damage; Retinal regeneration; DAPI; Quantum dot
Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES/Brazil)
- Funda do deAmparo a Pesquisa do Estado de Minas Gerais (FAPEMIG/Brazil)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq/Brazil)
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To evaluate DAPI (4',6-diamidino-2-phenylindole) as a nuclear tracer of stem cell migration and incorporation it was observed the pattern of retinal integration and differentiation of mesenchymal stem cells (MSCs) injected into the vitreous cavity of rat eyes with retinal injury, For this purpose adult rat retinas were submitted to laser damage followed by transplantation of DAPI-labeled BM-MSCs grafts and double-labeled DAPI and quantum dot-labeled BM-MSCs. To assess a possible DAPI diffusion as well its the integration and differentiation of DAPI-labeled BM-MSCs in laser-injured retina, host retinas were evaluated 8 weeks after injury/transplantation. It was demonstrated that. 8 weeks after the transplant, most of the retinal cells in all neural retinal presented nuclear DAPI labeling, specifically in the outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL). Meanwhile, at this point, most of the double-labeled BM-MSCs (DAPI and quantum dot) remained in the vitreous cavity and no retinal cells presented the quantum dot marker. Based on these evidences we concluded that DAPI diffused to adjacent retinal cells while the nanocrystals remained labeling only the transplanted BM-MSCs. Therefore, DAPI is not a useful marker for stem cells in vivo tracing experiments because the DAPI released from dying cells in moment of the transplant are taken tip by host cells in the tissue.
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